Cryptococcus laurentii (Kufferath) Skinner, anamorph 菌株拉丁名
(ATCC® 18803™) 菌株编号
Strain Designations 菌株别名 CBS 139 [CCRC 20527, CCY 17-3-2, DBVPG 6265, IFO 0609, IFO 0906, MUCL 30398, NRRL Y-2536, VKM Y-1665, VKPM Y-219]
Application 用途 Produces xylan endo-1,3-beta-xylosidase xylan hydrolase, xylanase
Quality control strain
Quality control strain for API products
Control strain for identification
Biosafety Level 生物安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 生长条件
Frozen 冷冻物 : -80℃ or colder
Freeze-Dried 冻干物 : 2℃ to 8℃
Live Culture 活菌 : See Propagation Section
Type Strain 模式菌株 yes
Preceptrol® yes
Comments 注释 Alkali-tolerant at pH 10
Morphology On YM medium at 25℃ after 7 days, colonies creamy white to dingy white, raised, smooth, margin entire. Cells subglobose to ellipsoidal, smooth, hyaline, 6-8 X 3-6 µm. Pseudohyphae not observed.
Medium 培养基 ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 336: Potato dextrose agar (PDA)
Growth Conditions 生长条件
Temperature 培养温度 : 24℃ to 26℃
Atmosphere 需氧情况 : Typical aerobic
Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence
GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAAAGATTGACCGAAAGGTCTTATCTCTATATCCCTC
ACCTCTGTGAACTGTGGACCTCCGGGTCTGTCTTAACAAACATCAGTGTAATGAACGTATAAATCATTAAA
CAAAACAAAACTTTCAACAACGGATCTCTTGGCTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGT
AATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCTTTTGGTATTCCGAAAGG
CATGCCTGTTTGAGTGTCATGAAATCTCAATCCCCCCGGGTTTATGATCTGGTCGGGACTTGGACATGGG
CGTCTGCCGGTCACACGGCTCGCCTCAAATGACTTAGTGGATCTCTCTGCATCCGTGACAGACGTAATA
AGTTTCGTCTTGTCCCTTGCGTACGAGTCCGCTCATAACCTGCCATCGCGCACTTTAGACTCTGACCTC
AAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAATAA D1D2
region of the 26S ribosomal RNA gene
ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAACCGGGAAGAGCTCA
AATTTGAAATCTGGCGTCCTCAGGGCGTCCGAGTTGTAATCTATAGAGGCGTTTTCCGTGCCGGACCGTGTC
CAAGTTCCTTGGAACAGGATATCAAAGAGGGTGACAATCCCGTACTTGACACGACGACCGGTGCTCTGTGA
TACGTCTTCTACGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGGTGGTGAGTTCCATCTAAAGCTAAATA
TTGGCGAGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGT
ACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGACCGAGAGGCTCAGCCGGTTCTGCCGGTGTAT
TCCCCTCGGTCGGGTCAACATCAGTTTTGTCCGGTGGATAAGGACGGTAGGAAGGTGGCACCCTCGGGTGTG
TTATAGCCTGCCGTCGCATACATCGGGTGAGACTGAGGAACGCAGCTCGCCTTTATGGCCGGGGTTCGCCCA
CGTCCGAGCTTAGGATGTTGACATAATGGCTTTAAACGAC
Morphology On YM medium at 25℃ after 7 days, colonies creamy white to dingy white, raised, smooth, margin entire. Cells subglobose to ellipsoidal, smooth, hyaline, 6-8 X 3-6 µm. Pseudohyphae not observed.
Name of Depositor 寄存人 CBS
Chain of Custody 来源国家 ATCC <-- CBS <-- H Kufferath
Isolation 分离源 Palm wine, malaffou, Congo
Cross References Nucleotide (GenBank) : EU266564 ITS including 5.8S rRNA gene
Nucleotide (GenBank) : KU729165 D1/D2 region of 26S rRNA gene
Nucleotide (GenBank) : AF075469 Cryptococcus laurentii 26S ribosomal RNA gene, partial sequence.
Nucleotide (GenBank) : AF218974 Cryptococcus laurentii strain ATCC 18803T 5.8S ribosomal RNA gene, partial sequence; internal transcribed spacer 2, complete sequnece; 28S ribosomal RNA gene, partial sequence.
References 参考文献 Sutherland ES, et al. Production of double stranded ribonucleic acid. US Patent 3,597,318 dated Aug 3 1971 Kufferath H. Peut-on obtenir du mout de biere alcalin?. Ann. Bull. Seances Soc. R. Sci. Med. Nat. Bruxelles 74: 16-46, 1920.
Biely P, et al. Xylan-degrading activity in yeasts: growth on xylose, xylan and hemicelluloses. Folia Microbiol. 23: 366-371, 1978.
Fell JW, et al. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evol. Microbiol. 50: 1351-1371, 2000. PubMed: 10843082
Aono R. Taxonomic distribution of alkali-tolerant yeasts. Syst. Appl. Microbiol. 13: 394-397, 1990.
API 20C AUX. bioMerieux. McTaggart LR, et al. Rapid identification of Cryptococcus neoformans and Cryptococcus gattii by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 49: 3050-3053, 2011. PubMed: 21653762
Yurkov AM, Golubev WI. Phylogenetic study of Cryptococcus laurentii mycocinogenic strains. Mycol Prog 12: 777-782, 2013.
Butler MI, Poulter RT. The PRP8 inteins in Cryptococcus are a source of phylogenetic and epidemiological information. Fungal Genet. Biol. 42: 452-463, 2005. PubMed: 15809009
Takashima M, et al. Three new combinations from the Cryptococcus laurentii complex: Cryptococcus aureus, Cryptococcus carnescens and Cryptococcus peneaus. Int. J. Syst. Evol. Microbiol. 53: 1187-1194, 2003. PubMed: 12892148
Scorzetti G, et al. Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res. 2: 495–517, 2002. PubMed: 12702266
Chen YC, et al. Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts. J. Clin. Microbiol. 39: 4042-4051, 2001. PubMed: 11682528
