Escherichia coli (Migula) Castellani and Chalmers 拉丁名
(ATCC® 47076™) 菌株编号
Classification Enterobacteriaceae, Escherichia
Strain Designations 菌株别名 MG1655
Application 用途 Transformation host Bacteriophage host
Biosafety Level 生物安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Shipping Information Frozen gycerol stock of E. coli
Storage Conditions 保藏条件 -80°C or colder
Preceptrol® no
Genotype F- lambda- Type
Strain no
Comments 注释 Electronic access to the correlations between the restriction map and the ordered phage library of Kohara are available over the Internet by anonymous ftp at these hosts: ncbi.nlm.nih.gov and utmmg.med.uth.tmc.edu.
Host strain with well-characterized restriction maps for NotI, SfiI, XbaI and BlnI. Can be used as a molecular size marker for pulsed-field gel electrophoresis.
AE000111 through AE000510 are individual GenBank numbers for the complete sequence, U00096 Derived from the parent strain W1485 by curing of the F plasmid.
Medium 培养基 ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions 生长条件
Temperature 培养温度 : 37°C
Atmosphere 需氧情况 : Aerobic
Genotype F- lambda-
Name of Depositor 寄存人 GM Weinstock
Chain of Custody 来源国家 ATCC <-- GM Weinstock <-- MG1655
References 参考文献 Blattner FR. Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res. 21: 5408-5417, 1993. PubMed: 8265357
Blattner FR, et al. The complete genome sequence of Escherichia coli K-12. Science 277: 1453-1462, 1997. PubMed: 9278503
Dicker IB. Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli. J. Bacteriol. 173: 334-344, 1991. PubMed: 1987124
Heath JD, et al. NotI genomic cleavage map of Escherichia coli K-12 strain MG1655. J. Bacteriol. 174: 558-567, 1992. PubMed: 1729244
Plunkett G III, et al. Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucleic Acids Res. 21: 3391-3398, 1993. PubMed: 8346018
Jensen KF. The Escherichia coli K-12 'wild types' W3110 and MG1655 have an rphframeshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. Gene 175: 3401-3407, 1993. PubMed: 8501045
Freddolino PL, Amini S, and S Tavazoie. Newly Identified Genetic Variations in Common Escherichia coli MG1655 Stock Cultures. J. Bacteriol. 194(2): 303-306, 2012. PubMed: 22081388
Cross References Nucleotide (GenBank) : U00096 complete sequence
Nucleotide (GenBank) : X72920 intercistronic region
Nucleotide (GenBank) : X64592 gene for ribosomal protein S7, partial
Nucleotide (GenBank) : L10328 chromosomal region from 81.5 to 84.5 minutes
Nucleotide (GenBank) : L19201 chromosomal region from 87.2 to 89.2 minutes
Nucleotide (GenBank) : M87049 chromosomal region from 84.5 to 86.5 minutes
Nucleotide (GenBank) : U00006 chromosomal region from 89.2 to 92.8 minutes
Nucleotide (GenBank) : X54797 firA gene, firA(200) and firA(201) allele, and skp gene (3' end)
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